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By Rodolfo Paoletti, Dr. David Kritchevsky

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T h e lipid droplets in the preparation chased for 2 minutes are labeled more heavily. Fig. 26, X 19,000; Fig. 27, X 26,000. (From O. Stein and Stein, 1968, reproduced by permission of the Editor of J. Cell Biol) 50 O. STEIN AND Y. STEIN Light and Electron Microscopic Radio autography ofLipids 51 the main site of triglycéride formation, but did not exclude the possible role of the outer mitochondrial membrane in that process. Another finding revealed by radioautography was the accumulation of label over lipid droplets with time of perfusion (Fig.

Stein and Stein (1969). The second limitation was that in order to obtain enough uptake during a relatively short pulse (5 minutes) for radioautography at the electron microscope level it was necessary to use choline-deficient rats in order to enhance incorporation into lecithin. Owing to the above constraints it was mandatory to supplement the radioautographic study of choline-labeled lecithin with data obtained by subcellular fractionation in which use could be made of other labels (linoleic acid, glycerol, lysolecithin) and of normal rats.

1969), 15 minutes after exposure of intestinal loops to a micellar solution containing oleic acid-3H. Thus the steps described in lipid transport in the intestine involve the entry of the substrate by physical diffusion, its esterification into Fig. 21. Section of hamster intestine, labeled for 5 minutes by incubation with oleic acid- 3 H and postincubated for 5 minutes in buffer. Grains are concentrated to the Golgi zones of the epithelial cells. X 4000. (From Dermer, 1968; reproduced by the courtesy of the author and by permission of the Editor of/.

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